Cell Based Assays
Promega Glutamine/Glutamate-Glo Assay, 50ml
The Glutamine/Glutamate-Glo Assay is a bioluminescent assay for rapid, selective and sensitive detection of glutamine and glutamate in biological samples. The assay is based on the conversion of glutamine to glutamate by Glutaminase enzyme.
Promega ERK1 Kinase Enzyme System, 1mg
Recombinant full-length, tag-free human ERK1 was expressed by baculovirus in Sf9 insect cells and activated by active MEK1 in vitro. ERK1 is a protein serine/threonine kinase that is a member of the extracellular signal-regulated kinases (ERKs).
Promega ADP-Glo™ Kinase Assay + CDK3/CyclinE1 Kinase Enzyme System
Recombinant full-length human CDK3 and Cyclin E1 were co-expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag on both proteins. CDK3/CyclinE1 is a member of the cyclin-dependent protein kinase family.
Promega NanoBRET TE Intracellular BET BRD Complete Kit, 1,000 assays
NanoBRET Target Engagement Intracellular BET BRD Assay measures compound binding at select BET bromodomain target proteins within intact cells. The assay is based on NanoBRET, an energy transfer technique for measuring molecular proximity in living cells.
Promega T Cell Activation Bioassay (IL-2), Propagation Model, 1 each
The T Cell Activation Bioassay, Propagation Model, provides a biologically relevant and specific MOA-based measure of TCR/CD3 Effector Cells without the complex workflow and variability inherent in primary cell-based assays.
Promega HDAC-Glo™ I/II Assay and Screening Systems
The HDAC-Glo™ I/II Assays and Screening System are single-reagent-addition, homogeneous, luminescent assays that measure the relative activity of histone deacetylase (HDAC) class I and II enzymes from cells, extracts or purified enzyme sources.
Promega Glucose Uptake-Glo Assay, 5ml
The Glucose Uptake-Glo Assay is a non-radioactive, plate-based, homogeneous bioluminescent method for measuring glucose uptake in mammalian cells based on the detection of 2-deoxyglucose-6-phosphate (2DG6P).
Promega ADP-Glo™ Kinase Assay + PKCθ Kinase Enzyme System
Recombinant full-length human PKC theta was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. Protein Kinase C theta (PKC theta) is an important component in the intracellular signaling cascade.
Promega PAK1/CDC42 Kinase Enzyme System, 1mg
Recombinant full-length human PAK1 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag and combined with CDC42 at a ratio of 1:4 (PAK1:CDC42) in vitro. PAK1 is a member of the p21-activated kinases (PAKs).
Promega ADP-Glo™ Kinase Assay + CK1α1 Kinase Enzyme System
Full-length recombinant human CK1 alpha 1 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. CK1 alpha 1 is a member of the CK1 family of serine/threonine protein kinases, which play an important role in diverse cell processes.
Promega ADP-Glo™ Kinase Assay + MLCK Kinase Enzyme System
Recombinant human mLCK (1425-1776) was expressed by baculovirus in Sf9 insect cells using a N-terminal GST tag. mLCK or myosin light chain kinase is a muscle member of the immunoglobulin gene superfamily and a calcium/calmodulin-dependent protein kinase.
Promega ADP-Glo™ Kinase Assay + TNIK Kinase Enzyme System
Recombinant human TNIK (1-367) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. TNIK or TRAF2 and NCK-interacting kinase are characterized by N-terminal kinase and C-terminal GCK domains that serve a regulatory function.
Promega MAPKAPK5 Kinase Enzyme System, 1mg
Recombinant full-length human MAPKAPK5 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. MAPKAPK5 is a member of the serine/threonine kinase family that responds to cellular stress and proinflammatory cytokines.
Promega ADP-Glo™ Kinase Assay + PAK1/CDC42 Kinase Enzyme System
Recombinant full-length human PAK1 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag and combined with CDC42 at a ratio of 1:4 (PAK1:CDC42) in vitro. PAK1 is a member of the p21-activated kinases (PAKs).
Promega CDK5/p35 Kinase Enzyme System, 10microg
Recombinant full-length human CDK5 and human p35 were co-expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. CDK5 is a member of the Cyclin-Dependent Kinase family that is most abundant in the mammalian brain.